The Agilent 2100 Bioanalyzer is a microfluidics-based platform offering qualitative and semi-quantitative analysis of DNA, RNA, proteins and cells. For an overview of the Agilent Bioanalyzer 2100 platform and product line, please visit their website.
The DNA Array Lab currently provides Agilent Bioanalyzer services on the 2100 Bioanalyzer for the RNA 6000 Nano LabChip and the DNA 7500 LabChip assays.
The RNA 6000 Nano LabChip assay measures quality of total and messenger RNA samples. Ribosomal fragment integrity, RNA fragment size and the presence of DNA contaminants can be identified prior to downstream use in sensitive applications like microarray analysis. More information on this assay is available from Agilent.
The DNA 7500 LabChip assay measures size and concentration for DNA fragments ranging in size from 100 to 7500 base pairs. Target DNA fragments and contaminants can be identified before use in downstream applications. More information on this assay is available from Agilent.
The following are instructions on how Fred Hutchinson and other Cancer Consortium members can access Agilent 2100 Bioanalyzer services at FHCRC. Those new to this technology or new to using the DNA Array Lab are strongly encouraged to meet with lab personnel prior to planning a project. For non-consortium members interested in services please contact the DNA Array Laboratory.
Contacts:
Samples must be submitted at a concentration of 50 to 500 ng/ul in a volume of no less than 3ul. When submitting samples, please indicate the type of sample material and organism.
Submission formsIf you are using a FHCRC project id for billing, please fill out the Internal Submission Form.
If billing is to be submitted to the University of Washington or other external institution, fill out the External submission form.
All samples and a fully completed sample submission form should be delivered to the DNA Array Lab, located in the Thomas Building, DE-740.
Data and electropherograms are generated with the Agilent 2100 Expert Software in the form of a .pdf document. This document presents data in peak tables, gel-like images and an assay-specific results table. Raw data files (.xad files), will be supplied upon request. In this case the researcher will need to download a version of the 2100 Expert Software from the Agilent website.
Data generated are transferred to servers supported by the Hutchinson Center's Research Computing Support Shared Resource. Data is transferred to the user's 'fred' account, in the researcher's 'dnaarray/bioanalyzer' folder.
If you do not currently have an account on the FRED server please fill out theFRED Account Application Form.
