Strictly adhering to FHCRC Genomics Resource standard procedures, the Genomics Resource Lab will add additional hybridization buffers when necessary and hybridize the labeled targets overnight to the array type requested. Following hybridization, the array undergoes post-hybridization washes as dictated by FHCRC Genomics Resource standard procedures
Once the array has been processed it is scanned by the Genomics Resource Lab to generate a TIFF image file. The lab is equipped with two GenePix 4000B scanners,which are used to acquire post hybridization microarray images. The software used to control the GenePix 4000B scanners (GenePix Pro 6.0) can also be used to visualize and extract quantitative data from saved microarray images in the format of TIFF files.
Data generated within the resource are transferred through automated pipelines to systems supported by the Hutchinson Center's Research Computing Support shared resource.
If you do not currently have an account on the Research Computing server, please fill out the User Account Application prior to the completion of your microarray experiment.
For more information on downstream data processing and storage please see our informatics web page.
Additionally, there are many third party software applications that can be used to further process and analyze your microarray data. Some of the software applications available to you can be found on the Genomics IT web page. (License agreements dictate that some software is available only from FHCRC computers. If you are not at FHCRC and need access to software, please contact Genomics and we will arrange access for you.)
Jeff Delrow, Ph.D.
Director and Staff Scientist, Genomics Resource
voice : 206.667.2763
email : jdelrow@fhcrc.org
Martin Morgan, Ph.D.
Director and Staff Scientist, Bioinformatics Shared Resource
voice: 208.667-2793
email: mtmorgan@fhcrc.org
 | |
| |
