Immunochemistry

Experimental histopathology offers extensive immunohistochemistry services. The laboratory uses automated immunochemistry stainers which offer consistency and standardized protocols on large and small scale projects.

Antibodies can be provided by the user or Experimental Histopathology laboratory. We have over 100 hundred antibody protocols worked up for a number of species. Please contact the lab for an updated list of antibodies.

We can use various detection methods including immunoperoxidase, alkaline phosphatase, and fluorescence. Protocols include dual IHC and multi-color IF. The staff has a great deal of experience with antigen retrieval techniques for formalin fixed tissue and with staining human and animal tissue.

• Many antibodies work in formalin fixed tissue, however, some do not. The staff can help you determine if you should fix your tissue and help you choose the best fixative.
• Many antibodies are not suitable for immunochemistry. The staff can help you identify the best antibody for your application.
• We may already have an immunochemistry protocol worked up for your protein of interest. See our list of antibodies.

Forms for Service

If you are using a FHCRC project id for billing, please fill out the Internal Submission Form and submit with your sample.

If billing is to be submitted to the University of Washington or other external institution, fill out the External Submission Form and submit with your sample.