Clonal Analysis

Location:	1100 Fairview Ave. N. Thomas Building, D1-327
Phone:	(206) 667-3369
Fax:	(206) 667-5978
E-mail:	mharkey@fhcrc.org
Contact:	Michael A. Harkey, Ph.D.
	Ludmila Golubev
	Deborah Higginbotham

The Clonal Analysis facility was established to provide VNTR (variable number tandem repeat), and LAM-PCR (linear amplification mediated PCR) and other molecular technology in support of transplantation research as well as basic research. The facility provides five primary functions:

1. Small-scale animal genomic DNA purification.
   High quality genomic DNA will be purified with Qiagen chemistry from up to 5X10⁶ cells or up to 50 mg soft tissue. DNA yield is up to 25 ug.



2. mRNA Isolation/cDNA synthesis from small cell populations.
   Robust cDNA template is generated for expression analysis from as few as 100 cells and up to 1X10⁷ cells. About 10 Expression assays can be performed from 100 cells.



3. Chimerism analysis using fluorescent VNTR.
   This technology quantifies the relative contributions of individual donors and recipients in a chimeric mix of cells.



4. Identification and tracking of retrovirally marked clones by LAM-PCR.
   This technology identifies clones, based on the unique insertion site of an integrated retrovirus.



5. Consulting on strategies and experimental design in clonal analysis.
   This category includes the design and implementation of a variety of molecular screening methods (please inquire).

The Core provides these functions on a user fee basis. For more information on pricing, see the User Fee listing.

	User Fees